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KMID : 0545120000100040482
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 4 p.482 ~ p.487
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Optimization of Submerged Culture Conditions for Exo-Biopolymer Production by Paecilomyces japonica
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BAE, JUN TAE
SINHA, JAYANTA/PARK, JONG PIL/SONG, CHI HYUN/YUN, JONG WON
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Abstract
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Optimization of submerged culture conditions for the production of exo-biopolymer from Paecilomyces japonica was studied. Maltose, yeast extract, and potassium phosphate were the most suitable sources of carbon, nitrogen, and inorganic salt, respectively, for both production of the exo-biopolymer and mycelial growth. The optimal culture conditions in a flask culture were pH 5.0, 25℃, and 150rpm in a medium containing (as in g/l) 30 maltose. 6 yeast extract, 2 polypeptone, 0.5 K_2HPO_4, 0.2 KH_2PO_4, 0.2MnSO_4·5H_2O, 0.2MgSO_4·7H_2O. Exo-biopolymer production and mycelial growth in the above suggested medium were significantly increased in a 2.5-1 jar fermentor, where the maximum biopolymer concentration was 8g/l. The morphological changes of the mycelium in the submerged culture were observed within pH ranges from 4.0 to 9.0; i.e., growth of the filamentous form was optimal at culture pHs of 5.0 and 6.0, whereas pellet was formed at other pHs.
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